It is important to make sure the protein therapeutics, such as recombinant proteins, peptides and monoclonal antibodies, are biosynthesized correctly. Peptide mapping works as a powerful analytical approach to confirm the identity of a specific protein, detail characterization of the protein& screen and identify PTMs. The purified proteins/peptides are reduced into short peptides by protease cleavage or chemical cleavage, then separated and analyzed with MALDI-TOF and ESI-TOF o identify the peptide sequence by peptide mass fingerprints. A cocktail of proteases usually are used to generate more unique peptides.  peptide mapping

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